Pflanzliche Entwicklungsbiologie




Original Paper


66) Rettenmaier R, Thieme N, Streubel J, Di Bello L, Kowollik ML, Huang L, Maus I, Klingl A, Liebl W, Zverlov VV (2021). Variimorphobacter saccharofermentans gen. nov., sp. nov., a new member of the family Lachnospiraceae, isolated from a maize-fed biogas fermenter. International Journal of Systematic and Evolutionary Microbiology 71(11). DOI: 10.1099/ijsem.0.005044

65) Ostermeier M, Heinz S, Hamm J, Zabret J, Rast A, Klingl A, Nowaczyk MM, Nickelsen J (2021). Thylakoid attachment to the plasma membrane in Synechocystis so. PCC 6803 requires the AncM protein. Plant Cell: koab253. DOI: 10.1093/plcell(koab253

64) Tůmová P, Voleman L, Klingl A, Nohýnková E, Wanner G, Doležal P (2021). Inheritance of the reduced mitochondria of Giardia intestinalis is coupled to the flagellar maturation cycle. BMC Biology 19(7): 193. DOI: 10.1186/s12915-021-01129-7

63) Rahlff J, Turzynski V, Esser SP, Monsees I, Bornemann TLV, Figueroa-Gonzalez PA, Schulz F, Woyke T, Klingl A, Moraru C, Probst AJ (2021). Lytic archaeal viruses infect abundant primary producers in Earth's crust. Nature Communications 12(1): 4642. DOI: 10.1038/s41467-021-24803-4

62) Garcia-Molina A, Lehmann M, Schneider K, Klingl A and Leister D (2021). Inactivation of cytosolic FUMARASE2 enhances growth and photosynthesis under simultaneous copper and iron deprivation in Arabidopsis. The Plant Journal 106: 766-784. DOI: 10.1111/tpj.15199

61) Beheshti H, Strotbek C, Asif Arif M, Klingl A, Top O, Frank W (2021). PpGRAS12 acts as a positive regulator of meristem formation in  Physcomitrium patens. Plant Molecular Biology. DOI: 10.1007/s11103-021-01125-z

60) Rettenmaier R, Kowollik M-L, Klingl A, Liebl W, Zverlov V (2021). Ruminiclostridium herbifermentans sp. nov., a mesophilic and moderately thermophilic cellulolytic and xylanolytic bacterium isolated from a lab-scale biogas fermenter fed with maize silage. International Journal of Systematic and Evolutionary Microbiology. DOI: 10.1099/ijsem.0.004692


59) Kaletta J, Pickl C, Griebler C, Klingl A, Kurmayer R and Deng L (2020). A rigorous assessment and comparison of enumeration methods for environmental viruses. Scientific Reports 10: 18625. DOI: 10.1038/s41598-020-75490-y

58) Chadha Y, Seydel C, and Klingl A (2020). Archaeal Cells. eLS 1: 224-239. DOI: 10.1002/9780470015902.10029201

57) Tůmová P, Nohýnková E, Klingl A, Wanner G (2020). A rapid workflow for the characterization of small numbers of unicellular eukaryotes by using correlative light and electron microscopy. Journal of Microbiological Methods 172: 105888. DOI: 10.1016/j.mimet.2020.105888

56) Flechsler J, Heimerl T, Pickl C, Rachel R, Stierhof Y-D, Klingl A (2020). 2D and 3D immunogold localization on (epoxy) ultrathin sections with and without osmium tetroxide. Microscopy Research & Technique 83: 691-705. DOI: 10.1002/jemt.23459

55) Schäfer K, Künzler P, Schneider KKlingl A, Eubel H, Carrie C (2020). The plant mitochondrial TAT pathway is essential for complex III biogenesis. Current Biology 30: 1-14. DOI: 10.1016/j.cub.2020.01.001

54) Kumar R, Tang Q, Müller SA, Gao P, Mahlstedt D, Zampagni S, Tan Y, Klingl A, Bötzel K, Lichtenthaler SF, Hüglinger GU, Koeglsperger T (2020). Fibroblast growth factor 2-mediated regulation of neuronal exosome release depends on CAMP3/cellubrevin in hippocampal neurons. Advanced Science 7: 1902372. DOI: 10.1002/advs.201902372


53) Schuster JA, Klingl A, Vogel RF, Ehrmann MA (2019). Polyphasic characterization of two novel Lactobacillus spp. isolated from blown salami packages: Description of Lactobacillus halodurans sp. nov. and Lactobacillus salsicarnum sp. nov. Systematic and Applied Microbiology 42(6): 126023. DOI: 10.1016/j.syapm.2019.126023

52) Mora M, Wink L, Kögler I, Mahnert A, Rettberg P, Schwendner P, Demets R, Cockell C, Alekhova T, Klingl A, Krause R, Zolotariof A, Alexandrova A, Moissl-Eichinger C (2019). Space Station conditions are selective but do not alter microbial characteristics relevant to human health. Nature Communications 10: 3990. DOI: 10.1038/s41467-019-11682-z

51) Gras S, Jimenez-Ruiz E, Klinger CM, Schneider K, Klingl A, Lemgruber L, Meissner M (2019). An endocytic-secretory cycle participates in Toxoplasma gondii in motility. PLoS Biology 17(6). e3000060. DOI: 10.1371/journal.pbio.3000060

50) Espinoza-Corral R, Heinz S, Klingl A, Jahns P, Lehmann M, Meurer J, Nickelsen J, Soll J, Schwenkert S (2019). Plastoglobular protein 18 is involved in chloroplast function and thylkoid formation. Journal of Experimental Botany 70(15): 3981-3993. DOI: 10.1093/jxb/erz177

49) Liang J, Klingl A, Lin YY, Boul E, Thomas-Oates J, Marín M (2019). A sub-compatible rhizobium strain reveals infection duality in Lotus. Journal of Experimental Botany 70(6): 1903-1913. DOI: 10.1093/jxb/erz057

48) Xu D, Marino G, Klingl A, Enderle B, Monte E, Kurth J, Hiltbrunner A, Leister D and Kleine T (2019). Extrachloroplastic PP7L functions in chloroplast development and abiotic stress tolerance. Plant Physiology 180/1): 323-341. DOI: 10.1104/pp.19.00070


47) Pfeiler S, Thakur M, Grünauer P, Megens RTA, Joshi U, Coletti R, Samara V, Müller-Stoy G, Ishikawa-Ankerhold H, Stark K, Klingl A, Fröhlich T, Arnold GJ, Wörmann S, Bruns CJ, Algül H, Weber C, Massberg S and Engelmann B (2018). CD36-triggered cell invasion and persistent tissue colonization by tumor microvesicles during metastasis. The FASEB Journal 33. DOI: 10.1096/fj.201800985R

46) Brameyer S, Plener L, Müller A, Klingl A, Wanner G and Jung K (2018). Outer membrane vesicles facilitate trafficking of the hydrophobic signaling molecule CAI-1 between Vibrio harveyi cells. Journal of Bacteriology 200: e00740-17. DOI: 10.1128/JB.00740-17

45) Probst AJ, Ladd B, Jarett JK, Geller-McGrath DE, Sieber CMK, Emerson JB, Anantharaman K, Thomas BC, Malmstrom RR, Stieglmeier M, Klingl A, Woyke T, Ryan MD and Banfield JF (2018). Differential depth distribution of microbial function and putative symbionts through sediment-hosted aquifers in the deep terrestrial subsurface. Nature Microbiology 3(3): 328-336. DOI: 10.1038/s41564-017-0098-y


44) Schieferbein F, Bauer M, Klingl A and Schopf S (2017). Mineral specific biofilm formation of Acidibacillus ferrooxidans Huett2. Solid State Phenomena 262: 334-338. DOI: 10.4028/

43) Forde AJ, Albrecht N, Klingl A, Donovan C and Bramkamp M (2017). Polymerization dynamics of the prophage-encoded actin-like protein AlpC is influenced by the DNA-binding adapter AlpA. Frontiers in Microbiology 8: 1429. DOI: 10.3389/fmicb.2017.01429

42) Poppleton DI, Duchateau M, Hourdel V, Matondo M, Flechsler J, Klingl A, Beloin C and Gribaldo S (2017). Outer membrane proteome of Veillonella parvula: A diderm firmicute of the human Microbiome. Frontiers in Microbiology 8: 1215. DOI: 10.3389/fmicb.2017.01215

41) Beck-Broichsitter M, Bohr A, Aragão-Santiago L, Klingl A and Kissel T (2016). Formulation and process considerations for the design of sildenafil-loaded polymeric microparticles by vibrational spray-drying. Pharmaceutical Development and Technology 22(6): 691-698. DOI: 10.3109/10837450.2015.1098661

40) Cerri MR, Wang Q, Stolz P, Folgmann J, Frances L, Katzer K, Li X, Heckmann AB, Wang T, Downie A, Klingl A, de Carvalho-Niebel F, Xie F and Parniske M (2017). The ERN1 transcription factor gene is a target of the CCaMK/CYCLOPS complex and controls rhizobial infection in Lotus japonicus. New Phytologist 215(1): 323-337. DOI: 10.1111/nph.14547

39) Schreiber V, Dersch J, Puzik K, Bäcker O, Liu X, Stork S, Schulz J, Heimerl T, Klingl A, Zauner S, Maier UG (2017). The central vacuole of the diatom Phaeodactylum tricornutum: Identification of new vacuolar membrane proteins and a functional di-leucine based targeting motif. Protist 168(3): 271-282. DOI: 10.1016/j.protis.2017.03.001

38) Zander A, Willkomm S, Ofer S, van Wolferen M, Egert L, Buchmeier S, Stöckl S, Tinnefeld P, Schneider S, Klingl A, Albers S-V, Werner F and Grohmann D (2017). Guide-independent DNA cleavage by archaeal Argonaute from Methanocaldococcus jannaschii. Nature Microbiology 2: 17034. DOI: 10.1038/nmicrobiol.2017.34

37) Rühl P, Pöll U, Braun J, Klingl A and Kletzin A (2017). A sulfur oxygenase from the haloalkaliphilic bacterium Thioalkalivibrio paradoxus with atypically low reductase adtivity. Journal of Bacteriology 199: e00675-16. DOI: 10.1128/JB.00675-16.


36) Antunes LCS, Poppleton D, Klingl A, Criscuolo A, Dupuy B, Brochier-Armanet C, Beloin C and Gribaldo S (2016). Phylogenomic analysis supports the ancestral presence of LPS-outer membranes in the Firmicutes. eLife 5: e14589. DOI: 10.7554/eLife.14589

35) Rast A, Rengstl B, Heinz S, Klingl A and Nickelsen J (2016). The role of Slr0151, a tetratricopeptide repeat protein from Synechocystis sp. PCC 6803, during photosysten II assembly and repair. Frontiers in Plant Science 7: 605. DOI: 10.3389/fpls.2016.00605


34) Dwarakanath S, Brenzinger S, Gleditzsch D, Plagens A, Klingl A, Thormann K and Randau L (2015). Interference activity of a minimal type I CRISPR-Cas system from Shewanella putrefaciens. Nucleic Acids Research 43(18): 8913-23. DOI: 10.1093/nar/gkv882

33) De Luca M, Ferraro MM, Hartmann R, Rivera-Gil P, Klingl A, Nazarenus M, Ramirez A, Parak WJ, Bucci C, Rinaldi R, and del Mercato LL (2015). Advances in use of capsule-based fluorescent sensors for measuring acidification of endocytic compartments in cells with altered expression of V-ATPase subunit V1G1. ACS Applied Materials & Interfaces 7(27): 15052-60. DOI: 10.1021/acsami.5b04375

32) Perras AK, Daum B, Ziegler C, Takahashi L, Ahmed M, Wanner G, Klingl A, Leitinger G, Kolb-Lenz D, Gribaldo S, Auerbach A, Mora M, Probst AJ, Bellack A and Moissl-Eichinger C (2015). S-layers at second glance? Altiarchaeal grappling hooks (hami) ressemble archaeal S-layer proteins in structure and sequence. Frontiers in Microbiology 6: 543. DOI: 10.3389/fmicb.2015.00543

31) Kletzin A, Heimerl T, Flechsler J, Van Niftrik L, Rachel R and Klingl A (2015). Cytochromes c in Archaea: distribution, maturation, cell architecture and the special case of Ignicoccus hospitalis. Frontiers in Microbiology 6: 439. DOI: 10.3389/ fmicb.2015.00439

30) Knierim C, Enzeroth M, Kaiser P, Dams C, Nette D, Seubert A, Klingl A, Greenblatt CL, Jérôme V, Agarwal S, Freitag R and Greiner A (2015). Living composites of bacteria and polymers as biomimetic films for metal sequestration and bioremediation. Macromolecular Bioscience 2015. DOI: 10.1002/mabi.201400538

29) Schuhmacher JS, Rossmann F, Dempwolff F, Knauer C, Altegoer F, Steinchen W, Dörrich AK, Klingl A, Stephan M, Linne U, Thormann KM, and Bange G (2015). MinD-like ATPase FlhG effects location and number of bacterial flagella during C-ring assembly. PNAS 112(10): 3092-7. DOI: 10.1073/pnas.1419388112

28) Lang K, Schuldes J, Klingl A, Daniel R, and Brune A (2015). Comparative genome analysis of “Candidatus Methanoplasma termitum” indicates a new mode of energy metabolism in the seventh order of methanogens. Applied and Environmental Microbiology 81(4): 1338-1352. DOI: 10.1128/AEM.03389-14

27) Losensky G, Vidakovic L, Klingl A, Pfeifer F and Fröls S (2015). Novel pili-like surface structures of Halobacterium salinarum strain R1 are crucial for surface adhesion. Frontiers in Microbiology 5: 755. DOI: 10.3389/fmicb.2014.00755


26) Klingl A (2014). S-layer and cytoplasmic membrane – exceptions from the typical archaeal cell wall with a focus on double membranes. Frontiers in Microbiology 5: 624. DOI: 10.3389/fmicb.2014.00624

25) Probst AJ, Weinmaier T, Raymann K, Perras A, Emerson JB, Rattei T, Wanner G, Klingl A, Berg IA, Yoshinaga M, Viehweger B, Hinrichs K-U, Thomas BC, Meck S, Auerbach AK, Heise M, Schintlmeister A, Schmid M, Wagner M, Gribaldo S, Banfield JF and Moissl-Eichinger C (2014). Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface. Nature Communications 5: 5497. DOI: 10.1038/ncomms6497

24) Meyer C, Heimerl T, Wirth R, Klingl A and Rachel R (2014). The Iho670 fibers of Ignicoccus hospitalis are anchored in the cell by a spherical structure located beneath the inner membrane. Journal of Bacteriology 196(21): 3807-3815. DOI: 10.1128/JB.01861-14

23) Perras AK, Wanner G, Klingl A, Mora M, Auerbach AK, Heinz V, Probst AJ, Huber H, Rachel R, Meck S and Moissl-Eichinger C (2014). Grappling archaea: ultrastructural analyses of an uncultivated, cold-loving archaeon, and its biofilm. Frontiers in Microbiology 5:397. DOI: 10.3389/fmicb.2014.00397

22) Stieglmeier M, Klingl A, Rittmann S, Alves RE, Melcher M, Leisch N and Schleper C (2014). Nitrososphaera viennensis sp. nov., an aerobic and mesophilic ammonia oxidizing archaeon from soil and member of the novel archaeal phylum Thaumarchaeota. International Journal of Systematic and Evolutionary Microbiology 64(8): 2738-52. DOI: 10.1099/ijs.0.063172-0

21) Klingl A (2014). Making new out of (g)old – Osmium and epon as standard for immunogold localization. Imaging & Microscopy 2/2014: 34-36.

20) Plagens A, Tripp V, Daume M, Sharma K, Klingl A, Hrle A, Conti E, Urlaub H and Randau L (2014). In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex. Nucleic Acids Research 42(8): 5125-5138. DOI: 10.1093/nar/ gku120

19) Siewering K, Jain S, Friedrich C, Webber-Birungi MT, Semchonok DA, Binzen I, Wagner A, Huntley S, Kahnt J, Klingl A, Boekema EJ, Søgaard-Andersen L and van der Does C (2014). The ubiquitous peptidoglycan-binding protein TsaP functions in surface assembly of type IV pili. PNAS 111(10): E953-61. DOI: 10.1073/pnas.1322889111

18) Van Teeseling MCF, de Almeida N, Klingl A, Speth DR, Op den Camp HJM, Rachel R, Jetten MSM and van Niftrik L (2014). A new addition to the cell plan of anammox bacteria: "Candidatus Kuenenia stuttgartiensis" has a protein surface layer as the outermost layer of the cell. Journal of Bacteriology 196(1): 80-89. DOI: 10.1128/JB.00988-13


17) Klingl A, Flechsler J, Heimerl T and Rachel R (2013). Archaeal Cells. In: eLS. John Wiley & Sons, Ltd:Chichester. DOI: 10.1002/9780470015902.a0000383.pub2

16) Peschke M, Moog D, Klingl A, Maier UG and Hempel F (2013). Evidence for glycoprotein transport into complex plastids. PNAS 110(26): 10860-10865. DOI: 10.1073/pnas. 1301945110


15) Reimann J, Lassak K, Khadouma S, Ettema TJG, Yang N, Driessen AJM, Klingl A and Albers SV (2012). Regulation of archaella expression by the FHA and von Willebrand Domain-containing proteins ArnA and ArnB in Sulfolobus acidocaldarius. Molecular Microbiology 86(1): 24-36.

14) Bubendorfer S, Held S, Windel N, Paulick A, Klingl A and Thormann K (2011). Specificity of motor components in the dual flagellar system of Shewanella putrefaciens CN-32. Molecular Microbiology 83(2): 335-350. DOI: 10.1111/j.1365-2958.2011.07934.x

13) Lassak K, Neiner T, Ghosh A, Klingl A, Wirth R and Albers SV (2011). Molecular analysis of the crenarchaeal flagellum. Molecular Microbiology 83(1): 110-124. DOI: 10.1111/j. 1365-2958.2011.07916.x.


12) Hempel F, Lau J, Klingl A and Maier UG (2011). Algae as protein factories: expression of a human antibody and the respective antigen in the diatom Phaeodactylum tricornutum. PLoS ONE 6(12): e28424. DOI:10.1371/journal.pone.0028424

11) Gonzalez NH, Felsner G, Klingl A, Schramm FD, Maier UG and Bolte K (2011). A single peroxisomal targeting signal mediates matrix protein import in diatoms. PLoS ONE 6(9): e25316. DOI:10.1371/journal.pone.0025316

10) Wu ML, van Teeseling MCF, Willems MJR, van Donselaar EG, Klingl A, Rachel R, Geerts WJC, Jetten MSM, Strous M and van Niftrik L (2011). Ultrastructure of the denitrifying methanotroph ‘Candidatus Methylomirabilis oxyfera’: a novel polygonal-shaped bacterium. Journal of Bacteriology 194(2): 284-291. DOI: 10.1128/JB.05816-11

9) Hempel F, Bozarth AS, Lindenkamp N, Klingl A, Zauner S, Linne U, Steinbüchel A and Maier UG (2011). Microalgae as bioreactors for bioplastic production. Microbial Cell Factories 10: 81. DOI:10.1186/1475-2859-10-81

8) Klingl A, Moissl-Eichinger C, Wanner G, Zweck J, Huber H, Thomm M and Rachel R (2011). Analysis of the surface proteins of Acidithiobacillus ferrooxidans strain SP5/1 and the new, pyrite-oxidizing Acidithiobacillus isolate HV2/2, and their possible involvement in pyrite oxidation. Archives of Microbioloy 193: 867-882. DOI:10.1007/s00203-011-0720-y

7) Zolghadr B, Klingl A, Rachel R, Driessen AJM and Albers SV (2011). The bindosome is a structural component of the Sulfolobus solfataricus cell envelope. Extremophiles 15: 235-244.


6) Rachel R, Meyer C, Klingl A, Gürster S, Heimerl T, Wasserburger N, Burghardt T, Küper U, Bellack A, Schopf S, Wirth R, Huber H and Wanner G (2010). Analysis of the Ultrastructure of Archaea by Electron Microscopy. Methods in Cell Biology 96: 47-69.

5) Loibl M, Grossmann G, Stradalova V, Klingl A, Rachel R, Tanner W, Malinsky J and Opekarová M (2010). C-terminus of Nce102 determines the structure and function of microdomains in the Saccharomyces cerevisiae plasma membrane. Eukaryotic Cell 9: 1184-1192.

4) Zolghadr B, Klingl A, Koerdt A., Driessen AJM, Rachel R and Albers SV (2010). Appendage-Mediated Surface Adherence of Sulfolobus solfataricus. Journal of Bacteriology 192: 104-110.


3) Veith A, Klingl A, Zolghadr B, Lauber K, Mentele R, Lottspeich F, Rachel R, Albers SV and Kletzin A (2009). Acidianus, Sulfolobus and Metallosphaera Surface Layers: Structure, Composition and Gene Expression. Molecular Microbiology 73: 58-72.


2) Etzel K, Klingl A, Huber H, Rachel R, Schmalz G, Thomm M and Depmeier W (2008). Etching of {111} and {210} synthetic pyrite surfaces by two archaeal strains, Metallosphaera sedula and Sulfolobus metallicus. Hydrometallurgy 94: 116-120.


1) Klingl A, Thomm M, Menath V, Huber H, Rachel R, Etzel K, Toporski J, Depmeier W and Schmalz G (2007). Ultrastructural Aspects of Microbes Etching Pyrite Surfaces. Microscopy and Microanalysis 13(Suppl. 03): 212-213. DOI: 10.1017/ S1431927607081068

Book Chapter

5) Blohs M, Moissl-Eichinger C, Mahnert A, Spang A, Dombrowski N, Krupovic M, Klingl A (2019). Archaea – An Introduction. In: Schmidt TM (ed.): Encyclopedia of Microbiology, 4th Edition, 243-252, Academic Press.

4) Klingl A, Pickl C, Flechsler J (2019) Archaeal Cell Walls. In: Kuhn A. (eds) Bacterial Cell Walls and Membranes. Subcellular Biochemistry, vol 92. Springer, Cham. DOI: 10.1007/978-3-030-18768-2_14

3) Albers SV, Pohlschroder M, eds. (2016). Archaeal Cell Envelope and Surface Structures. Lausanne: Frontiers Media. DOI: 10.3389/978-2-88919-773-6 

2) Comolli LR, Luef B, Auer M, eds. (2015). Intra- and inter-species interactions in microbial communities. Lausanne: Frontiers Media. DOI: 10.3389/978-2-88919-449-0

1) Hempel F, Bolte K, Klingl A, Zauner S and Maier UG (2014). Protein transport into plastids of secondarily evolved organisms. Chapter 11. In: Plastid Biology. Advances in Plant Biology 5: 291-303. Springer Science & Business Media, New York, 2014. DOI: 10.1007/978-1-4939-1136-3_11

Other publications

3) Braun V, Fuchs G, Schloter M, Schubert T, Graw J, Kruck D, Sander J, Drews G, Schild S, Hantke K, Wiegand S, Westermann A, Klingl A, Jungfer K (2020). Journal Club. BIOspektrum 26(1): 56-62. DOI: 10.1007/s12268-020-1318-1

2) Monsees I, Klingl A, and Probst AJ (2019). Kleine Zellen, große Wirkung – Bakterien der Candidate Phyla Radiation. BIOspektrum 25: 719-721. DOI: 10.1007/s12268-019-1304-7

1) Flechsler J, Pickl C and Klingl A (2019). Eine oder zwei Membranen bei Prokaryoten: Was war zuerst da? BIOspektrum 25(1): 29-31. DOI: 10.1007/s12268-019-0999-9